This study is NOT currently recruiting participants.
Number
11-E-0006
Sponsoring Institute
National Institute of Environmental Health Sciences (NIEHS)
Recruitment Detail
Type: Completed Study; data analyses ongoing Gender: Male & Female Min Age: 18 Years Max Age: 60 Years
Referral Letter Required
No
Population Exclusion(s)
Children;Pregnant Women;Fetuses
Keywords
TGF-Beta; Asthma; Natural History
Recruitment Keyword(s)
Healthy Volunteer; Asthma; Respiratory Syncytial Virus; RSV
Condition(s)
Asthma
Investigational Drug(s)
None
Investigational Device(s)
Intervention(s)
Supporting Site
National Institute of Environmental Health Sciences
- Human respiratory syncytial virus (RSV) is a virus that causes respiratory tract infections, and is frequently responsible for hospital visits in infants and children. It can also trigger severe breathing problems for individuals who have asthma, but these infections are generally better tolerated in non-asthmatics. Some research suggests that lack of an efficient immune system response in people with asthma may make it difficult for the body to fight the effects of RSV.
- Transforming Growth Factor-beta (TGF-[beta]) is a chemical in the body that is more prevalent in the lungs of people with asthma and related respiratory disorders. More information is needed about the effects of TGF-[beta] and whether it makes individuals with asthma more prone to developing RSV. Researchers hope to use this information to determine possible treatments and therapies for individuals with asthma who contract RSV.
Objectives:
- To determine the possible role of TGF-[beta] in increased asthmatic susceptibility to RSV infection.
Eligibility:
- Individuals between 18 and 60 years of age who are either healthy nonsmokers or mild asthmatics.
Design:
- This study involves a screening visit and a study visit.
- Participants will be screened with a medical history and physical examination, as well as blood samples and a pulmonary function test.
- At the study visit, participants will receive mild anesthetic and have a bronchoscopy, in which researchers insert a bronchoscope through the participant s nose or mouth and into the lungs to examine the lungs and collect lung cells.
- Participants will be contacted by a research team member 24 36 hours after the bronchoscopy to ask about any side effects from the procedure.
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INCLUSION CRITERIA: 1. Male or female between 18 and 60 years of age 2. Non-asthmatics and mild asthmatics as defined below: -Non-asthmatic must have no prior diagnosis of asthma, no history of health care utilization or medication use for asthma, no current symptoms, and Forced Expiratory Volume in 1 Second (FEV1) greater than or equal to 80% predicted. -Asthmatics must have physician-diagnosed asthma for at least one year and evidence of mild, persistent disease during the month prior to Visit 1 (based on 2008 GINA guidelines). a) Participants who are currently taking a controller medication must: i. use the equivalent to GINA Step 1 or 2 therapy; and ii. have controlled disease, as defined by: -daytime symptoms less than or equal to 2 times week, such as wheezing, tightness in the chest, shortness of breath, and cough, - no nocturnal symptoms; b) Participants who are not currently taking a controller must have: i.<TAB>daytime symptoms > 1 time a week but < 1 time a day, such as wheezing, tightness in the chest, shortness of breath, and cough; ii. nocturnal awakenings > 2 times a month but < 1 time a week; c) All asthmatic participants must have either: i.<TAB>Pre-bronchdilator FEV1 greater than or equal to 80% predicted and a positive methacholine challenge (PC20 less than or equal to 4 mg / ml); or ii.<TAB>Pre-bronchodilator FEV1 < 80% and post-bronchodilator FEV1 greater than or equal to 80% with significant bronchodilator reversibility (at least 12% or 200ml change in FEV1) 3. Participants must be able to understand and provide written informed consent 4. Participants must be able to travel to the CRU and the EPA EXCLUSION CRITERIA: 1. Use of oral steroid treatment(s) within 30 days of Visit 1 2. Acute asthma-related healthcare utilization within 30 days of Visit 1, such as ED visits, systemic corticosteroids, and nebulizer treatment for asthma exacerbation 3. Known or suspected respiratory infections within 30 days of Visit 1, such as flu, pneumonia, severe cold, tuberculosis, or bronchitis 4. Known or suspected viral infection within 30 days of Visit 1 5. History of chronic obstructive pulmonary disease other than asthma 6. History of immunological disease or current cancer 7. Uncontrolled cardiovascular disease such as angina, prior myocardial infarction, stroke, and high cholesterol 8. Cardiac malformations 9. Pulmonary hypertension 10. Bleeding disorders 11. Facial deformity, major facial surgery 12. Currently pregnant or breast feeding 13. Current smoker, significant second-hand smoke exposure (defined by urine cotinine > 100 ng/ml at Visit 1 or Visit 2) or former smokers (defined by a history of smoking > 100 cigarettes) 14. Insulin dependent diabetes 15. Used any of the following medications within 30 days of Visit 1: oral corticosteroids, systemic immunosuppressants or other immune-modifying drugs [e.g., Rituxan, Humira, Enbrel, Azathioprine (Imuran), Cyclosporine (Neoral, Sandimmune, and SangCya), cyclophosphamide, TNF antagonists], anticoagulants (clopidogrel, heparin, enoxaparin and related drugs, coumadin), and sustained use (i.e. more than one dose per day for more than two days) of non-steroidal anti-inflammatory drugs (aspirin, ibuprofen, indomethacin) within seven days of bronchoscopy 16. Allergy or history of adverse reactions to methacholine or lidocaine 17. Severe asthma as defined by a history of intubation for unstable asthma 18. Any condition that, in the investigator's opinion, places the participant at undue risk for complications (e.g. from PFTs, fiberoptic bronchoscopy, bronchalveolar lavage and/or bronchial brushings) 19. Temperature > 37.6 C; blood pressure < 90/50 mm Hg or blood pressure >170/95 mm Hg; pulse rate < 50 or > 100 beats/minute 20. Body weight < 50 kg (<110 lbs) 21. The following abnormal lab values (values obtained during clinical assessment): - Platelet count < 100,000 per 10(9)/L - White blood cells count < 3000 per 10(9)/L - Hematocrit < 35% for both female and male - Prothrombin Time (PT) / Abnormal International Normalized Ratio (INR) and partial prothromboplastin time (PTT) based on reference laboratory established reference ranges - Serum creatinine > 1.4 mg/dL
1. Male or female between 18 and 60 years of age
2. Non-asthmatics and mild asthmatics as defined below:
-Non-asthmatic must have no prior diagnosis of asthma, no history of health care utilization or medication use for asthma, no current symptoms, and Forced Expiratory Volume in 1 Second (FEV1) greater than or equal to 80% predicted.
-Asthmatics must have physician-diagnosed asthma for at least one year and evidence of mild, persistent disease during the month prior to Visit 1 (based on 2008 GINA guidelines).
a) Participants who are currently taking a controller medication must:
i. use the equivalent to GINA Step 1 or 2 therapy; and
ii. have controlled disease, as defined by:
-daytime symptoms less than or equal to 2 times week, such as wheezing, tightness in the chest, shortness of breath, and cough,
- no nocturnal symptoms;
b) Participants who are not currently taking a controller must have:
i.<TAB>daytime symptoms > 1 time a week but < 1 time a day, such as wheezing, tightness in the chest, shortness of breath, and cough;
ii. nocturnal awakenings > 2 times a month but < 1 time a week;
c) All asthmatic participants must have either:
i.<TAB>Pre-bronchdilator FEV1 greater than or equal to 80% predicted and a positive methacholine challenge (PC20 less than or equal to 4 mg / ml); or
ii.<TAB>Pre-bronchodilator FEV1 < 80% and post-bronchodilator FEV1 greater than or equal to 80% with significant bronchodilator reversibility (at least 12% or 200ml change in FEV1)
3. Participants must be able to understand and provide written informed consent
4. Participants must be able to travel to the CRU and the EPA
EXCLUSION CRITERIA:
1. Use of oral steroid treatment(s) within 30 days of Visit 1
2. Acute asthma-related healthcare utilization within 30 days of Visit 1, such as ED visits, systemic corticosteroids, and nebulizer treatment for asthma exacerbation
3. Known or suspected respiratory infections within 30 days of Visit 1, such as flu, pneumonia, severe cold, tuberculosis, or bronchitis
4. Known or suspected viral infection within 30 days of Visit 1
5. History of chronic obstructive pulmonary disease other than asthma
6. History of immunological disease or current cancer
7. Uncontrolled cardiovascular disease such as angina, prior myocardial infarction, stroke, and high cholesterol
8. Cardiac malformations
9. Pulmonary hypertension
10. Bleeding disorders
11. Facial deformity, major facial surgery
12. Currently pregnant or breast feeding
13. Current smoker, significant second-hand smoke exposure (defined by urine cotinine > 100 ng/ml at Visit 1 or Visit 2) or former smokers (defined by a history of smoking > 100 cigarettes)
14. Insulin dependent diabetes
15. Used any of the following medications within 30 days of Visit 1: oral corticosteroids, systemic immunosuppressants or other immune-modifying drugs [e.g., Rituxan, Humira, Enbrel, Azathioprine (Imuran), Cyclosporine (Neoral, Sandimmune, and SangCya), cyclophosphamide, TNF antagonists], anticoagulants (clopidogrel, heparin, enoxaparin and related drugs, coumadin), and sustained use (i.e. more than one dose per day for more than two days) of non-steroidal anti-inflammatory drugs (aspirin, ibuprofen, indomethacin) within seven days of bronchoscopy
16. Allergy or history of adverse reactions to methacholine or lidocaine
17. Severe asthma as defined by a history of intubation for unstable asthma
18. Any condition that, in the investigator's opinion, places the participant at undue risk for complications (e.g. from PFTs, fiberoptic bronchoscopy, bronchalveolar lavage and/or bronchial brushings)
19. Temperature > 37.6 C; blood pressure < 90/50 mm Hg or blood pressure >170/95 mm Hg; pulse rate < 50 or > 100 beats/minute
20. Body weight < 50 kg (<110 lbs)
21. The following abnormal lab values (values obtained during clinical assessment):
- Platelet count < 100,000 per 10(9)/L
- White blood cells count < 3000 per 10(9)/L
- Hematocrit < 35% for both female and male
- Prothrombin Time (PT) / Abnormal International Normalized Ratio (INR) and partial prothromboplastin time (PTT) based on reference laboratory established reference ranges
- Serum creatinine > 1.4 mg/dL
Principal Investigator
Referral Contact
For more information: