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Protocol Details

Collection of Human Biospecimens for Basic and Clinical Research into Globin Variants

This study is currently recruiting participants.

Summary | Eligibility | Citations | Contacts

Summary

Number

19-I-0093

Sponsoring Institute

National Institute of Allergy and Infectious Diseases (NIAID)

Recruitment Detail

Type: Participants currently recruited/enrolled
Gender: Male & Female
Min Age: 18 Years
Max Age: 70 Years

Referral Letter Required

No

Population Exclusion(s)

Children;
Pregnant Women

Keywords

Assay Development;
Malaria;
Sickle Cell Disease;
Alpha and Beta Thalassemia;
Natural History

Recruitment Keyword(s)

None

Condition(s)

Alpha and Beta Thalassemia;
Sickle Cell Disease;
Malaria;
Human physiology

Investigational Drug(s)

None

Investigational Device(s)

None

Intervention(s)

None

Supporting Site

National Institute of Allergy and Infectious Diseases

Background:

Blood disorders like sickle cell disease and malaria affect many people around the world. Researchers want to learn more about blood disorders. To do this, they need to collect biological samples from people with blood disorders. They also need to collect samples from healthy people.

Objective:

To collect samples to use for research on blood disorders.

Eligibility:

People ages 18-70 who have blood disorders. Healthy volunteers without blood disorders are also needed.

Design:

Participants will be screened with a medical history, physical exam, and blood and urine tests.

Participants will give one or more samples. They will give them over 5 years. They can choose not to give any of the samples:

Saliva: Participants will spit into a tube. They may also have the inside of their mouth swabbed.

Urine: Participants will urinate into a cup.

Blood: Blood will be taken through a needle in the participant s arm.

Fat samples: An area on the participant s belly or buttock will be numbed. A small cut will be made into the skin and a small piece of fat removed.

Mucus and cells from the lungs: The participant will be sedated. A flexible tube will be inserted through the nose or mouth into the lung airways. These participants will also have a physical exam, chest x-ray, and heart tests after the procedure.

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Eligibility

PARTICIPANT INCLUSION CRITERIA:

1. Aged 18-70 years.

2. Able to provide informed consent.

3. Willing to allow biological samples to be stored for future research.

4. Willing to provide one or more of the following tissues: saliva, urine, blood, adipose tissue, bronchial brushing, and/or BAL samples.

5. Willing to allow genetic testing on collected biological samples.

PARTICIPANT EXCLUSION CRITERIA:

-Exclusion Criteria for Saliva Sample Collection Only

Any condition that, in the opinion of the principal investigator (PI), contraindicates participation in this study, will be cause for exclusion. However, there are no explicit exclusion criteria for the collection of saliva samples only.

-Exclusion Criteria for All Other Participants

The following exclusion criteria apply to all participants who will provide any of the following samples in-person at the NIH CC: urine, blood, adipose tissue, bronchial brushing, and/or BAL samples:

1. Pregnancy.

2. Positive testing for hepatitis B virus, hepatitis C virus, or HIV (as determined by serum screening tests or relevant viral quantitative studies.)

3. Any condition that requires active medical intervention or monitoring to avert serious danger to the individual s health or wellbeing.

4. Any condition that, in the opinion of the PI, contraindicates participation in this study.

-Additional Exclusion Criteria for Individuals Giving Blood for Research

1. Hemoglobin < 10 g/dL for healthy female volunteers, < 12 g/dL for healthy male volunteers, or < 6 g/dL for participants with sickle cell disease or other chronic anemias.

-Additional Exclusion Criteria for Adipose Tissue Biopsy

Individuals meeting any of the following criteria will be excluded from undergoing adipose tissue biopsy. If the participant no longer meets any of these criteria at a later time, then they will be allowed to undergo this procedure.

1. Currently taking anticoagulation medication.

2. Platelets < 100,000/microL.

3. History of keloid formation (or irregular fibrous tissue formed at the site of a scar or injury).

4. History of adverse reactions to lidocaine or other local anesthetics.

Use of aspirin (or acetylsalicylic acid) and nonsteroidal anti-inflammatory drugs (NSAIDs) are permitted.

-Additional Exclusion Criteria for Bronchoscopy

Individuals meeting any of the following criteria will be excluded from undergoing bronchoscopy. If the participant no longer meets any of these criteria at a later time, then they will be allowed to undergo this procedure.

1. Prothrombin time (PT) > 1 second above the upper limit of normal (ULN) or international normalized ratio > 1.3.

2. Partial thromboplastin time (PTT) > 1 second above ULN.

3. Platelets < 150,000/microL.

4. Currently taking anticoagulation medication.

5. Use of aspirin within 2 weeks of the bronchoscopy or NSAIDs within 2 days of the bronchoscopy.

6. Diagnosis of a pulmonary disorder (eg, asthma, chronic bronchitis, cystic fibrosis, or bronchiectasis).

7. Respiratory tract infection within the last 4 weeks.

8. History of adverse reactions to lidocaine or other local anesthetics.

9. History of cigarette smoking within the past 3 months.

10. History of chronic opioid use.

11. History of drug or alcohol abuse.

12. Post-bronchodilator FEV1 < 40% of predicted or pre-bronchodilator FEV1 < 35% of predicted.

13. Active bronchospasm on physical examination.

14. History of lidocaine allergy.

15. Any condition that, in the opinion of the PI, contraindicates this procedure.

Co-enrollment guidelines: Participants may be co-enrolled in other studies. However, the PI must be notified of co-enrollment.


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Citations:

Embury SH, Dozy AM, Miller J, Davis JR Jr, Kleman KM, Preisler H, Vichinsky E, Lande WN, Lubin BH, Kan YW, Mentzer WC. Concurrent sickle-cell anemia and alpha-thalassemia: effect on severity of anemia. N Engl J Med. 1982 Feb 4;306(5):270-4.

Piel FB, Weatherall DJ. The -thalassemias. N Engl J Med. 2014 Nov 13;371(20):1908-16. doi: 10.1056/NEJMra1404415.

Straub AC, Lohman AW, Billaud M, Johnstone SR, Dwyer ST, Lee MY, Bortz PS, Best AK, Columbus L, Gaston B, Isakson BE. Endothelial cell expression of haemoglobin regulates nitric oxide signalling. Nature. 2012 Nov 15;491(7424):473-7. doi: 10.1038/nature11626. Epub 2012 Oct 31.

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Contacts:

Principal Investigator

Referral Contact

For more information:

Amy P. Ruhl, M.D.
National Institute of Allergy and Infectious Diseases (NIAID)
NIHBC 10 - CRC BG RM B3-4207
10 CENTER DR
BETHESDA MD 20892
(240) 669-5776
parker.ruhl@nih.gov

Mary J. Jackson, R.N.
National Institute of Allergy and Infectious Diseases (NIAID)
Malaria Genetics Section
Building 10-CRC, Room B3-420, NIAID/NIH
Bethesda, MD 20814
(301) 761-5667
alpha.study@nihi.gov

Office of Patient Recruitment
National Institutes of Health Clinical Center (CC)
Building 61, 10 Cloister Court
Bethesda, Maryland 20892
Toll Free: 1-800-411-1222
Local Phone: 301-451-4383
TTY: TTY Users Dial 7-1-1
ccopr@nih.gov

Clinical Trials Number:

NCT03937817

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