INCLUSION CRITERIA:
Ability to sign informed consent.
Healthy male or female between 18-65 years of age.
Willing to have samples stored for future research.
EXCLUSION CRITERIA:
History of an immune deficiency, such as HIV infection or cancer.
History of chronic hepatitis B and/or C infection.
History of chronic anemia.
A known clotting disorder or taking medications that interfere with blood clotting, such as: aspirin, heparin, or Coumadin*
Current pregnancy.
History of allergy to lidocaine.
Have taken an investigational drug in the last 6 months.
Apheresis in the past 6 weeks.
Any other medical condition that the investigator feels puts the participant at too high a risk for participation.
* Coumadin and/or heparin treatment will not be stopped so that a subject can participate in this protocol. If Coumadin or heparin was recently discontinued, a subject can be enrolled, but no bone marrow procedures will be performed until the prothrombin time is less than 15.2 seconds and the partial thromboplastin time is within normal range. For other agents that interfere with blood clotting without prolonging the prothrombin time, a 7-day washout period will be required before bone marrow sampling.
(Procedure) EXCLUSION CRITERIA
Hemoglobin less than 12.3 mg/dl.
Platelets less thank 100, 000/mm(3).
PT greater than 15.2 seconds or PTT greater than 37.3 seconds
Positive b-HCG.
EKG changes suggestive of cardiovascular disease.
Any other lab value which may put the subject at risk during the procedure.
National Institutes of Health Clinical Center
Bethesda, Maryland 20892. Last update: 11/25/2009